GeneTex

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United States (US)

Biotin antibody

Cat. No. GTX21227

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, EM, IHC, Multiplexing

Reactivity

 Species independent
Package
100 μl ($399)
See all Biotin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000-1:25000
ICC/IF Assay dependent
ELISA 1:100000
EM Assay dependent
IHC Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM Sodium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

78 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Biotin conjugated to KLH

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. Anti-Biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

Research Area

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399