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United States (US)

Biotin antibody

Cat No. GTX21227

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000 - 1:25000
ELISA 1:100000
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

78 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Purification

Antiserum
Unpurified polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. Anti-Biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

Research Area

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339