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Blocking Buffer, TBS with BSA (10X)

Cat No. GTX48882

Application

WB, ELISA, IHC
Package
25 ml ($89)
PRODUCT

Summary

This product is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X TBS for initial testing. This product is usually more effective than nonfat milk for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Upon receipt store product at 4ºC. Product is shipped at ambient temperature.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WB Blocking Buffer , TBS with 1% BSA , TBS with BSA , TBS with bovine serum albumin

Background

GeneTex's BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.

These blocking buffers are 10% (w/v) solutions of high-quality BSA that are useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) for initial testing, but other buffer concentrations can be beneficial for specific systems. BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.

The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. Because individual blocking buffers are not compatible with every system, a variety of blockers in both Tris-buffered saline (TBS) and phosphate-buffered saline (PBS) are available. The best blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding.

To optimize the blocking step for a particular immunoassay, empirical testing is essential. Using inadequate amounts of blocker will result in excessive background. Using an excessive blocker concentration can mask antibody-antigen interactions or inhibit the marker enzyme. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.

Features and Benefits:
‧ Purified protein – 10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions
‧ Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications
‧ Easy to use – no waiting for powder to dissolve with this ready-to-dilute liquid concentrate
‧ Flexible – available in PBS and TBS formulations to suit a variety of applications

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Package List Price ($)
$ 89