GeneTex
United States (US)

Bmi1 antibody

GTX102020_41101_20171117_WB_2_19011506_147.jpg
GTX102020_41101_20171117_WB_19011506_157.jpg
GTX102020_41101_IP_19011506_361.jpg

Cat No. GTX102020

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bmi1 Proto-Oncogene, Polycomb Ring Finger,Flvi2/Bmi1,Pcgf4,Rnf51,Flvi-2/Bmi-1,Bmi1

Cellular Localization

Nucleus , Cytoplasm

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Database

Research Area

DATA IMAGES
GTX102020_41101_20171117_WB_2_19011506_147.jpg

GTX102020 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX102020) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102020_41101_20171117_WB_19011506_157.jpg

GTX102020 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX102020) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102020_41101_IP_19011506_361.jpg

GTX102020 IP Image

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments.
IP samples: K562 whole cell extract
A. 40 μg K562 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody (GTX102020)
5 % SDS-PAGE
The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX102020) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169