GeneTex
  • Country / Location Selection

United States (US)

Bmi1 antibody

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008
Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008
Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

Cat No. GTX114008

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116 , K562 , THP-1 , THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7

Predict Reactivity

Rat, Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036353

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Cellular Localization

Nucleus , Cytoplasm

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008

GTX114008 ICC/IF Image

BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX114008

GTX114008 IP Image

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 μg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects Bmi1 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Bmi1 antibody Cat No. GTX114008

Rating ( Average 4.6 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.6 based on 5 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2016.PMID: 27863490
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2016.PMID: 27863490
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2017.PMID: 28629331
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2015.PMID: 26136074
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169