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Bmi1 antibody

KO/KD Orthogonal
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX114008
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008
Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

Cat. No. GTX114008

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Bmi1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1 , 293T , A549 , H1299 , HCT116

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036353

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Cellular Localization

Nucleus , Cytoplasm

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects Bmi1 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008

GTX114008 ICC/IF Image

BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX114008

GTX114008 IP Image

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 μg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008

GTX114008 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114008

GTX114008 ICC/IF Image

Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Bmi1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114008

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2016.PMID: 27863490

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2016.PMID: 27863490

Anti-Bmi1 antibody used in Western Blot (WB). GTX114008

GTX114008 WB Image

Orthogonal

The data was published in the journal BMC Cancer in 2017.PMID: 28629331

REFERENCE

Application Reference

YC Hsu et al. Cancer Genomics Proteomics 2024; 21 (5) : 523-532 Targeting Bmi1 for Enhancing Anoikis Sensitivity and Inhibiting Metastasis in Colorectal Cancer
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Chien CH et al. J Biomed Sci 2019; 26 (1) : 77 Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets.
Application : WB
Reactivity : Human
Lai YJ et al. J Cell Physiol 2018; (Epub) CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1) : 14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Bmi1 antibody Cat. No. GTX114008

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-Bmi1 antibody used in Western Blot (WB). GTX114008
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
YC Hsu et al. Cancer Genomics Proteomics 2024; 21 (5):523-532 Targeting Bmi1 for Enhancing Anoikis Sensitivity and Inhibiting Metastasis in Colorectal Cancer
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Chien CH et al. J Biomed Sci 2019; 26 (1):77 Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets.
Application : WB
Reactivity : Human
Lai YJ et al. J Cell Physiol 2018; (Epub) CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1):14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Application : WB
Reactivity : Human
Huang CF et al. J Exp Clin Cancer Res 2017; 36 (1):116 NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Mouse
Wang Y et al. J Mol Neurosci 2017; (Epub) Endothelial Cells Promote Formation of Medulloblastoma Stem-Like Cells via Notch Pathway Activation.
Application : WB
Reactivity : Human
Huynh N et al. BMC Cancer 2017; 17 (1):431 Depletion of p21-activated kinase 1 up-regulates the immune system of APC14/+ mice and inhibits intestinal tumorigenesis.
Application : WB
Reactivity : Mouse
Ko CY et al. Mol Neurobiol 2017; (Epub) MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.
Application : WB
Reactivity : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Hsu CC et al. J Biomed Sci 2016; 23 (1):81 Suberoylanilide hydroxamic acid represses glioma stem-like cells.
Ju SY et al. Cell Death Dis 2015; 6 (Epub):e1801 identification of * as a novel therapeutic agent that targets human colon cancer stem cells.
Application : WB
Reactivity : Human
Chen YK et al. Int J Oral Sci 2014; 6 (4):212-8 Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169