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Bmi1 antibody

Anti-Bmi1 antibody used in Western Blot (WB). GTX32472
Anti-Bmi1 antibody used in Western Blot (WB). GTX32472
Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX32472

Cat. No. GTX32472

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Bmi1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody used in Western Blot (WB). GTX32472

GTX32472 WB Image

WB analysis of various sample lysates using GTX32472 Bmi1 antibody.
Dilution : 1:5000
Loading : 25μg per lane

Anti-Bmi1 antibody used in Western Blot (WB). GTX32472

GTX32472 WB Image

WB analysis of various sample lysates using GTX32472 Bmi1 antibody.
Dilution : 1:5000
Loading : 25μg per lane

Anti-Bmi1 antibody used in Immunoprecipitation (IP). GTX32472

GTX32472 IP Image

IP analysis of K-562 cell lysate using GTX32472 Bmi1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399