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Bmi1 antibody

Anti-Bmi1 antibody used in Western Blot (WB). GTX45791
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45791
Anti-Bmi1 antibody used in Western Blot (WB). GTX45791
Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45791

Cat. No. GTX45791

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Multiplexing

Reactivity

 Human
Package
50 μg ($399)
See all Bmi1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:13000
ICC/IF 1:200
ELISA 1:5000-1:30000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Cellular Localization

Nucleus,Cytoplasm

Background

The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody used in Western Blot (WB). GTX45791

GTX45791 WB Image

Western blot using GeneTex's Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 μg of a U2OS whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4º C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45791

GTX45791 ICC/IF Image

Immunofluorescence using affinity purified goat anti Bmi1(GTX45791) shows nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4ºC and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).

Anti-Bmi1 antibody used in Western Blot (WB). GTX45791

GTX45791 WB Image

WB analysis of U2OS whole cell lysate using GTX45791 Bmi1 antibody.
Loading : 20 μg
Dilution : 1:1000

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX45791

GTX45791 ICC/IF Image

ICC/IF analysis of MeOH-fixed HepG2 cells using GTX45791 Bmi1 antibody.
Green : Primary antibody
Red : Wheat germ agglutinin
Blue : DAPI
Dilution : 1:200

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399