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Bmi1 antibody

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48576
Anti-Bmi1 antibody used in Western Blot (WB). GTX48576

Cat No. GTX48576

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat, Zebrafish, Rabbit, Bovine, Cat, Chicken, Primate
Package
50 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 μg/ml
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Cellular Localization

Nucleus,Cytoplasm

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Database

Research Area

DATA IMAGES

Anti-Bmi1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48576

GTX48576 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48576 Bmi1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

Anti-Bmi1 antibody used in Western Blot (WB). GTX48576

GTX48576 WB Image

WB analysis of human skeletal muscle tissue lysate using GTX48576 Bmi1 antibody.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf