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Bordetella pertussis Toxin antibody

Anti-Bordetella pertussis Toxin antibody used in ELISA (ELISA). GTX64098
Anti-Bordetella pertussis Toxin antibody used in ELISA (ELISA). GTX64098
Anti-Bordetella pertussis Toxin antibody used in Western Blot (WB). GTX64098

Cat. No. GTX64098

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, Dot, ELISA, Neutralizing/Inhibition

Reactivity

 Bordetella pertussis
Package
100 μl ($399)
See all Bordetella pertussis Toxin products

APPLICATION

Application Note

Western blotting (1:2000-1:10000 dilution), ELISA (1:10000-1:20000 dilution), Dot blotting (1:2000-1:10000 dilution), Immunoprecipitation (1:200-1:500 dilution)

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Immunization was Initiated with toxoid and boosted with native toxin

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Bordetella pertussis Toxin antibody used in ELISA (ELISA). GTX64098

GTX64098 ELISA Image

Titration of pertussis toxin by direct ELISA using anti-pertussis toxin antiserum ELISA plate is coated with indicated amounts of pertussis toxin per well. Antiserum was used at 1/12,500 dilution. ELISA was performed as in Fig.2. Dynamic range was 100 pg to 10 ng under these conditions.

Anti-Bordetella pertussis Toxin antibody used in ELISA (ELISA). GTX64098

GTX64098 ELISA Image

Titration of antibody reactivity of anti-Pertussis antiserum by direct ELISA Plate was coated with 100 μg of pertussis toxin per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate.

Anti-Bordetella pertussis Toxin antibody used in Western Blot (WB). GTX64098

GTX64098 WB Image

Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western blotting using anti-perussis toxin antibody. 1. Culture medium of Bordetera pertussis. SDS-PAGE, silver-stained. 2. Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained. 3. Western blot of culture medium of Bordetera pertussis as in 1. 4. Western blot of purified pertussis toxin (10 ng) The toxin consists of five subunits as indicated by S1 to S5.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399