Bordetella pertussis Toxin antibody

Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western blotting using anti-perussis toxin antibody. 1. Culture medium of Bordetera pertussis. SDS-PAGE, silver-stained. 2. Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained. 3. Western blot of culture medium of Bordetera pertussis as in 1. 4. Western blot of purified pertussis toxin (10 ng) The toxin consists of five subunits as indicated by S1 to S5.

Titration of antibody reactivity of anti-Pertussis antiserum by direct ELISA Plate was coated with 100 μg of pertussis toxin per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate.

Titration of pertussis toxin by direct ELISA using anti-pertussis toxin antiserum ELISA plate is coated with indicated amounts of pertussis toxin per well. Antiserum was used at 1/12,500 dilution. ELISA was performed as in Fig.2. Dynamic range was 100 pg to 10 ng under these conditions.