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Borrelia burgdorferi Dbp-A antibody

Anti-Borrelia burgdorferi Dbp-A antibody used in Western Blot (WB). GTX48809
Anti-Borrelia burgdorferi Dbp-A antibody used in Western Blot (WB). GTX48809

Cat. No. GTX48809

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Borrelia burgdorferi
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA 1:5000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Borrelia burgdorferi

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A protein.

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Borrelia burgdorferi Dbp-A antibody used in Western Blot (WB). GTX48809

GTX48809 WB Image

Western blot showing detection of 0.1 μg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa). Lane 3: DbpA, MBP removed by TEV cleavage. Lane 4: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Anti-Borrelia burgdorferi Dbp-A antibody used in Western Blot (WB). GTX48809

GTX48809 WB Image

WB analysis of various samples using GTX48809 Borrelia burgdorferi Dbp-A antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-Dbp-A fusion protein
Lane 3 : Dbp-A protein
Lane 4 : MBP
Loading : 0.1 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399