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Borrelia burgdorferi Erpn/Ospe antibody

Anti-Borrelia burgdorferi Erpn/Ospe antibody used in Western Blot (WB). GTX48807

Cat No. GTX48807

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Borrelia burgdorferi
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ELISA 1:5,000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi ErpN/OspE protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

This product is antibody made against ErpN (OspE/F-Related Protein N), from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection.

Database

Research Area

DATA IMAGES

Anti-Borrelia burgdorferi Erpn/Ospe antibody used in Western Blot (WB). GTX48807

GTX48807 WB Image

Western blot showing detection of 0.1 μg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow; 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf