WB analysis of various samples using GTX48804 Borrelia burgdorferi Osp A antibody.
Lane 1 : Protein ladder
Lane 2 : 50 ng Osp A
Lane 3 : 20 ng Osp A
Lane 4 : 10 ng Osp A
Lane 5 : Blank
Lane 6 : 50 ng Osp B
Lane 7 : 50 ng Osp C
Dilution : 1:1000

WB analysis of various samples using GTX48804 Borrelia burgdorferi Osp A antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-Osp A fusion protein
Lane 3 : MBP
Loading : 0.1 μg
Dilution : 1:1000

Western blot showing detection of 0.1 μg of recombinant OspA protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspA fusion protein (arrow; expected MW: 70.5 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).