GeneTex
  • Country / Location Selection

United States (US)

Bovine STAT3 protein (active)

Bovine STAT3 protein (active). GTX00076-pro
Bovine STAT3 protein (active). GTX00076-pro
Bovine STAT3 protein (active). GTX00076-pro

Cat No. GTX00076-pro

Application

Functional Assay

Species

Bovine
Package
10 μg ($279)

APPLICATION

Application Note

Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT protein family. In response to cytokines and growth factors, STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. The protein can mediate the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. STAT3 is essential for the differentiation of the TH17 helper T cells, which have been implicated in a variety of autoimmune diseases. Besides, Glucose 7 Phosphate Isomerase (GPI) has been identified as an interactor of STAT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant bovine STAT3 and recombinant human GPI. Briefly, STAT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GPI-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-STAT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of STAT3 and GPI was in a dose dependent manner.

Observed MW

19 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val563~Leu670 (NP_001012689.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Bovine STAT3 protein (active). GTX00076-pro

GTX00076-pro Functional Assay Image

Functional ELISA analysis of GTX00076-pro Bovine STAT3 protein (active) which can bind immobilized GPI protein.

Bovine STAT3 protein (active). GTX00076-pro

GTX00076-pro Image

SDS-PAGE analysis of GTX00076-pro Bovine STAT3 protein (active).

Bovine STAT3 protein (active). GTX00076-pro

GTX00076-pro Image

WB analysis of GTX00076-pro Bovine STAT3 protein (active).

REFERENCE

There are currently no references for Bovine STAT3 protein (active) (GTX00076-pro). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Bovine STAT3 protein (active) (GTX00076-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 279