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Brachyury antibody [6C12]

Anti-Brachyury antibody [6C12] used in ELISA (ELISA). GTX60616
Anti-Brachyury antibody [6C12] used in Western Blot (WB). GTX60616
Anti-Brachyury antibody [6C12] used in Flow cytometry (FACS). GTX60616

Cat. No. GTX60616

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C12

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all Brachyury products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Brachyury (AA: 218-352) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box transcription factor T , SAVA , T , TFT

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-Brachyury antibody [6C12] used in ELISA (ELISA). GTX60616

GTX60616 ELISA Image

ELISA analysis of antigen using GTX60616 Brachyury antibody [6C12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Brachyury antibody [6C12] used in Western Blot (WB). GTX60616

GTX60616 WB Image

WB analysis of HEK293 (1) and Brachyury (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate using GTX60616 Brachyury antibody [6C12].

Anti-Brachyury antibody [6C12] used in Flow cytometry (FACS). GTX60616

GTX60616 FACS Image

FACS analysis of A549 cells using GTX60616 Brachyury antibody [6C12].
Green : Brachyury
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409