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Brachyury antibody

Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX131339
Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX131339
Anti-Brachyury antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131339

Cat. No. GTX131339

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Brachyury products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the centers region of human Brachyury. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886464

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box transcription factor T , SAVA , T , TFT

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX131339

GTX131339 IHC-P Image

Brachyury antibody detects Brachyury protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Brachyury antibody (GTX131339) diluted at 1:2000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Brachyury antibody used in IHC (Paraffin sections) (IHC-P). GTX131339

GTX131339 IHC-P Image

Brachyury antibody detects Brachyury protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Brachyury stained by Brachyury antibody (GTX131339) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Brachyury antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131339

GTX131339 ICC/IF Image

Brachyury antibody detects Brachyury protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Brachyury stained by Brachyury antibody (GTX131339) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169