Brachyury antibody detects Brachyury protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membranes were blotted with Brachyury antibody (GTX133714) diluted at 1:1000 and competitor's antibody (sc-17743) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Brachyury antibody detects Brachyury protein at nucleus in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Brachyury antibody (GTX133714) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min