GeneTex
United States (US)

Bradykinin B1 receptor antibody, Internal

GTX88647_2204_WB_18121411_472.jpg

Cat No. GTX88647

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2-4μg/ml
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88647-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence KVWELYKQCTPK, from the internal region of the protein sequence according to NP_000701.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bradykinin Receptor B1,B1Bkr,B1R,Bdkrb2,Bkb1R,Bkr1,Bradyb1,Bdkrb1

Background

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
GTX88647_2204_WB_18121411_472.jpg

GTX88647 WB Image

WB analysis of K562 lysate using GTX88647 Bradykinin B1 receptor antibody, Internal.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329