GeneTex
United States (US)

Bradykinin antibody

Cat No. GTX14391

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ELISA
Reactivity Human
APPLICATION

Application Note

ELISA: Use at a dilution of 1/250. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

72 kDa. ( Note )

Specificity/Sensitivity

Bradykinin: 100%
PROPERTIES

Form

Liquid

Buffer

Neat serum without preservatives.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

antiserum

Antigen Species

Human

Immunogen

Full length protein (Human) conjugated to BSA.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kininogen 1,Bdk,Bk,Hmwk,Kng,Kng1

Cellular Localization

Secreted

Background

Bradykinin is a nine-amino-acid vasoactive peptide which elicits numerous physiologic responses such as vasodilation, smooth muscle spasm and pain. Bradykinin is one of a family of such peptides called the kinins. These kinins are generated by proteolysis of high-molecular-weight precursors called kininogens upon activation by pathophysiologic conditions such as inflammation or allergy. The physiological actions of these kinins are brought about through their interaction with transmembrane receptors. There are two distinct bradykinin receptor subtypes; B1 and B2, both of which appear to be coupled to G-proteins. The B2 receptor subtype is found in healthy smooth muscle cells and neurons, whereas the B1 receptors are only detected following tissue injury. The B2 receptor is similar in structure to other seven-helix G-protein-coupled receptors. Bradykinin has relatively low affinity for B1 receptors, interacting primarily with the B2 receptor through which several second messenger systems are stimulated, including inositol phospholipid hydrolysis, arachidonic acid metabolism, tyrosine phosphorylation, and membrane depolarization and hyperpolarization.

Database

Research Area

Package List Price ($)
$ 319