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BrdU antibody [BU6-4]

Anti-BrdU antibody [BU6-4] used in Flow cytometry (FACS). GTX12219

Cat. No. GTX12219

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BU6-4

Isotype

 IgG1

Application

 ICC/IF, FACS, ELISA

Reactivity

 Species independent
Package
50 μg ($399)
See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Assay dependent
ELISA 5μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.4, 1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Bromodeoxyuridine-BSA conjugate.

Conjugation

Unconjugated

RRID

AB_368640

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-BrdU antibody [BU6-4] used in Flow cytometry (FACS). GTX12219

GTX12219 FACS Image

The data was published in the journal PLoS One in 2017.PMID: 28358913

REFERENCE

Application Reference

Ligasova A et al. PLoS One 2017; 12 (3) : e0174893 Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication.
Ligasova A et al. PLoS One 2015; 10 (7) : e0132393 The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2'-Deoxyuridine in Cellular DNA under Various Conditions.
Application : ICC/IF
Liboska R et al. PLoS One 2012; 7 (12) : e51679 Most anti-BrdU antibodies react with 2'-deoxy-5-ethynyluridine -- the method for the effective suppression of this cross-reactivity.
Application : ICC/IF
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SDS
PBS.pdf
Package List Price ($)
$ 399