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BrdU antibody [MoBu-1]

Anti-BrdU antibody [MoBu-1] used in Flow cytometry (FACS). GTX28039
Anti-BrdU antibody [MoBu-1] used in IHC (Paraffin sections) (IHC-P). GTX28039

Cat. No. GTX28039

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MoBu-1

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Species independent
Package
100 μg ($399)
See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_385364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-BrdU antibody [MoBu-1] used in Flow cytometry (FACS). GTX28039

GTX28039 FACS Image

FACS analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM cells using GTX28039 BrdU antibody [MoBu-1]. The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

Anti-BrdU antibody [MoBu-1] used in IHC (Paraffin sections) (IHC-P). GTX28039

GTX28039 IHC-P Image

IHC-P analysis of bromodeoxyuridine-labeled cells (chick embryo) using GTX28039 BrdU antibody [MoBu-1].

REFERENCE

Application Reference

Tu J et al. Sichuan Da Xue Xue Bao Yi Xue Ban 2023; 54 (5) : 965-971 [Photobiomodulation Promotes Hippocampal Neurogenesis and Improves Cognitive Function and Anti-Inflammatory Injury in Rats With Chronic Cerebral Hypoperfusion].
Sihan Guo et al. Front Cell Neurosci 2021; 15 : 731855 Photobiomodulation Promotes Hippocampal CA1 NSC Differentiation Toward Neurons and Facilitates Cognitive Function Recovery Involving NLRP3 Inflammasome Mitigation Following Global Cerebral Ischemia.
Application : IHC-Fr
Liu YY et al. PLoS One 2017; 12 (11) : e0187637 Hypoxia-preconditioned mesenchymal stem cells ameliorate ischemia/reperfusion-induced lung injury.
Huang TF et al. Am J Sports Med 2013; 41 (5) : 1117-25 Mesenchymal stem cells from a hypoxic culture improve and engraft Achilles tendon repair.
Application : ICC/IF

Clone Reference

Stanek D et al. Eur J Cell Biol 2000; 79 (3):202-7 Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399