BrdU antibody [MoBu-1]

Cat No. GTX28039

Host	Mouse
Clonality	Monoclonal
Clone Name	MoBu-1
Isotype	IgG1
Application	ICC/IF, IHC-P, FACS

See all BrdU products

Datasheet
Reference

APPLICATION

Application Note

IHC-P:Assay dependent
FACS:1-2 μg/ml
ICC/IF::2 μg/ml.
Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The reaction shows a clear, nuclear confined speckled pattern.

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine (isolated from Helix pomatia ).

Purification

Protein A purified
>95 % (by PAGE).

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Immunocytochemical labeling with the antibody provides a simple and rapid method for estimation of the cell proliferation. The antibody can be used for histochemistry on frozen tissue sections, formalin-fixed, paraffin-embedded sections as well as in immunofluorescence and FACS.

Research Area

- Epitope Tags & Reporters

DATA IMAGES

GTX28039 IHC-P Image

Immunohistochemistry of paraffin-embedded sections (chick embryo) using GTX28039.

GTX28039 FACS Image

Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

REFERENCE