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BrdU antibody [MoBu-1]

Anti-BrdU antibody [MoBu-1] used in IHC (Paraffin sections) (IHC-P). GTX28039
Anti-BrdU antibody [MoBu-1] used in Flow cytometry (FACS). GTX28039

Cat. No. GTX28039

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MoBu-1

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Species independent
Package
100 μg ($399)
See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_385364

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-BrdU antibody [MoBu-1] used in IHC (Paraffin sections) (IHC-P). GTX28039

GTX28039 IHC-P Image

IHC-P analysis of bromodeoxyuridine-labeled cells (chick embryo) using GTX28039 BrdU antibody [MoBu-1].

Anti-BrdU antibody [MoBu-1] used in Flow cytometry (FACS). GTX28039

GTX28039 FACS Image

FACS analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM cells using GTX28039 BrdU antibody [MoBu-1]. The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

REFERENCE

Application Reference

Sihan Guo et al. Front Cell Neurosci 2021; 15 : 731855 Photobiomodulation Promotes Hippocampal CA1 NSC Differentiation Toward Neurons and Facilitates Cognitive Function Recovery Involving NLRP3 Inflammasome Mitigation Following Global Cerebral Ischemia.
Application : IHC-Fr
Liu YY et al. PLoS One 2017; 12 (11) : e0187637 Hypoxia-preconditioned mesenchymal stem cells ameliorate ischemia/reperfusion-induced lung injury.
Huang TF et al. Am J Sports Med 2013; 41 (5) : 1117-25 Mesenchymal stem cells from a hypoxic culture improve and engraft Achilles tendon repair.
Application : ICC/IF

Clone Reference

Stanek D et al. Eur J Cell Biol 2000; 79 (3):202-7 Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399