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BrdU antibody

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091
Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

Cat No. GTX128091

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Positive Control

HeLa cells were treated with 100uM BrdU for 4hrs , *U343 cells were treated with 10 μM BrdU for 18hrs ,

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.01 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to BrdU

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168976

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) at nucleus in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU antibody (GTX128091) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

GTX128091 ICC/IF Image

BrdU antibody detects BrdU at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

REVIEW

BrdU antibody Cat No. GTX128091

Rating ( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-Dec-2015
Score :
Application Tested : IHC-Fr
Sample : zebrafish intestine mucosa tissue
Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : ICC/IF
Sample : U343 cells
Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr
Fixation : Methanol:Aceton (1:1)
Primary Antibody : 1:100, 4°C, 15Hr
Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169