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BrdU antibody

Orthogonal
Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091
Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

Cat No. GTX128091

Reference 7
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Package
100 μl ($329),
25 μl ($169)
See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Positive Control

HeLa cells were treated with 100uM BrdU for 4hrs , *U343 cells were treated with 10 μM BrdU for 18hrs ,

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to BrdU

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) at nucleus in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU antibody (GTX128091) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

GTX128091 ICC/IF Image

Orthogonal

BrdU antibody detects BrdU at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

Application Reference

Liu G et al. Int J Biol Sci 2019; 15 (7) : 1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Guosheng Liu et al. Int J Biol Sci 2019; 15 (7) : 1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Li HY et al. J Agric Food Chem 2019; 67 (7) : 1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Ni YL et al. Chem Biol Interact 2018; 291 : 137-143 Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.
Application : IHC
Zhu B et al. Cell Mol Life Sci 2018; (Epub) Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.
Application : IHC
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REVIEW

BrdU antibody Cat No. GTX128091

Rating
( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-BrdU antibody used in IHC (Frozen sections) (IHC-Fr). GTX128091
Date : Anonymous submitted on 29-Dec-2015
Score :
Application Tested : IHC-Fr
Sample : zebrafish intestine mucosa tissue
Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : ICC/IF
Sample : U343 cells
Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr
Fixation : Methanol:Aceton (1:1)
Primary Antibody : 1:100, 4°C, 15Hr
Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169