BrdU antibody

Name: BrdU antibody
Brand: GeneTex
SKU: GTX128091
Price: 329.00 USD
Rating: 4 (2 reviews)

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

Cat No. GTX128091

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Package	100 μl ($329), 25 μl ($169)

See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
FACS	1:50-1:200
IHC	Assay dependent

Not tested in other applications.

Positive Control

HeLa cells were treated with 100uM BrdU for 4hrs , *U343 cells were treated with 10 μM BrdU for 18hrs ,

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.01 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to BrdU

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168976

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) at nucleus in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU antibody (GTX128091) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX128091 ICC/IF Image

BrdU antibody detects BrdU at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

Application Reference

Lazarchuk P et al. Mol Cell Biol 2020; (Epub) Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells.

Liu G et al. Int J Biol Sci 2019; 15 (7) : 1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.

Guosheng Liu et al. Int J Biol Sci 2019; 15 (7) : 1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.

Li HY et al. J Agric Food Chem 2019; 67 (7) : 1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.

Ni YL et al. Chem Biol Interact 2018; 291 : 137-143 Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.

Application : IHC

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Submit a Reference

REVIEW

BrdU antibody Cat No. GTX128091

Rating

( Average 4 based on 2 users reviews)

Application

Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)		( Average 4 based on 1 users reviews)

Anti-BrdU antibody used in IHC (Frozen sections) (IHC-Fr). GTX128091

Date : Anonymous submitted on 29-Dec-2015

Score :

Application Tested : IHC-Fr

Sample : zebrafish intestine mucosa tissue

Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr

Fixation : 4% PFA

Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 21-Oct-2013

Score :

Application Tested : ICC/IF

Sample : U343 cells

Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr

Fixation : Methanol:Aceton (1:1)

Primary Antibody : 1:100, 4°C, 15Hr

Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.

Submit a Review

Application Reference

Liu G et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.

Guosheng Liu et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.

Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.

Ni YL et al. Chem Biol Interact 2018; 291:137-143 Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.

Application : IHC

Zhu B et al. Cell Mol Life Sci 2018; (Epub) Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.

Application : IHC

Sun J et al. Brain Res 2013; 1491:225-35 WIN55, 212-2 promotes differentiation of oligodendrocyte precursor cells and improve remyelination through regulation of the phosphorylation level of the ERK 1/2 via cannabinoid receptor 1 after stroke-induced demyelination.

Application : IHC

Stegeman H et al. Radiother Oncol 2013; 106 (3):383-9 Predictive value of hypoxia, proliferation and tyrosine kinase receptors for EGFR-inhibition and radiotherapy sensitivity in head and neck cancer models.