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BrdU antibody

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091
Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

Cat. No. GTX128091

Reference 13
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IHC

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all BrdU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to BrdU

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4oC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU stained by BrdU antibody (GTX128091) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

GTX128091 ICC/IF Image

BrdU antibody detects BrdU protein at nucleus and mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

W Shi et al. Neuroscience 2023; S0306-4522 (23) : 00265-8 Hypoxic Postconditioning Promotes Angiogenesis After Ischemic Stroke
Application : ICC/IF
Chen SY et al. Mol Ther Methods Clin Dev 2022; 26 : 157-168 Amelioration of experimental tendinopathy by lentiviral CD44 gene therapy targeting senescence-associated secretory phenotypes.
Application : IHC-P
Lanzafame M et al. Nucleic Acids Res 2021; 49 (19) : 10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Hattori Y et al. Biochem Pharmacol 2021; 186 : 114495 Attenuation of growth hormone production at the fetal stage is critical for dioxin-induced developmental disorder in rat offspring.
H?rdtner C et al. Basic Res Cardiol 2020; 115 (6) : 78 Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering.
Application : IHC-Fr
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Submit a Reference

REVIEW

BrdU antibody Cat. No. GTX128091

Rating
( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-BrdU antibody used in IHC (Frozen sections) (IHC-Fr). GTX128091
Date : Anonymous submitted on 29-Dec-2015
Score :
Application Tested : IHC-Fr
Sample : zebrafish intestine mucosa tissue
Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : ICC/IF
Sample : U343 cells
Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr
Fixation : Methanol:Aceton (1:1)
Primary Antibody : 1:100, 4°C, 15Hr
Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.
Submit a Review
Application Reference
W Shi et al. Neuroscience 2023; S0306-4522 (23):00265-8 Hypoxic Postconditioning Promotes Angiogenesis After Ischemic Stroke
Application : ICC/IF
Chen SY et al. Mol Ther Methods Clin Dev 2022; 26:157-168 Amelioration of experimental tendinopathy by lentiviral CD44 gene therapy targeting senescence-associated secretory phenotypes.
Application : IHC-P
Lanzafame M et al. Nucleic Acids Res 2021; 49 (19):10911-10930 Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation.
Hattori Y et al. Biochem Pharmacol 2021; 186:114495 Attenuation of growth hormone production at the fetal stage is critical for dioxin-induced developmental disorder in rat offspring.
H?rdtner C et al. Basic Res Cardiol 2020; 115 (6):78 Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering.
Application : IHC-Fr
Lazarchuk P et al. Mol Cell Biol 2020; (Epub) Mutual balance of histone deacetylases HDAC1, HDAC2, and the acetyl reader ATAD2 regulates the level of acetylation of histone H4 on nascent chromatin of human cells.
Liu G et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Guosheng Liu et al. Int J Biol Sci 2019; 15 (7):1383-1395 P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Ni YL et al. Chem Biol Interact 2018; 291:137-143 Teroxirone motivates apoptotic death in tumorspheres of human lung cancer cells.
Application : IHC
Zhu B et al. Cell Mol Life Sci 2018; (Epub) Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.
Application : IHC
Sun J et al. Brain Res 2013; 1491:225-35 WIN55, 212-2 promotes differentiation of oligodendrocyte precursor cells and improve remyelination through regulation of the phosphorylation level of the ERK 1/2 via cannabinoid receptor 1 after stroke-induced demyelination.
Application : IHC
Stegeman H et al. Radiother Oncol 2013; 106 (3):383-9 Predictive value of hypoxia, proliferation and tyrosine kinase receptors for EGFR-inhibition and radiotherapy sensitivity in head and neck cancer models.
Application : IHC-Fr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169