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BrdU antibody

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091
Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091
Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

Cat. No. GTX128091

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P, IHC-Fr, FACS, IHC

Reactivity

Species independent
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to BrdU

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-BrdU antibody used in Flow cytometry (FACS). GTX128091

GTX128091 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4oC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-BrdU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128091

GTX128091 ICC/IF Image

BrdU antibody detects BrdU protein at nucleus and mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BrdU antibody used in IHC (Paraffin sections) (IHC-P). GTX128091

GTX128091 IHC-P Image

BrdU antibody detects Bromodeoxyuridine (BrdU) in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU stained by BrdU antibody (GTX128091) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

BrdU antibody Cat. No. GTX128091

Rating ( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-Dec-2015
Score :
Application Tested : IHC-Fr
Sample : zebrafish intestine mucosa tissue
Blocking : 5% normal goat serum, 0.5% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5%Triton X-100 in PBS/ 2 N HCl and 10 mM citric acid buffer
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 21-Oct-2013
Score :
Application Tested : ICC/IF
Sample : U343 cells
Blocking : PBS + 0,5% BSA +0,5% Glycin, 20°C, 2Hr
Fixation : Methanol:Aceton (1:1)
Primary Antibody : 1:100, 4°C, 15Hr
Figure Note : U343 cells which were cultivated in DMEM medium + stimulation with 10% FCS for 24 and subsequent treatment with 10 μM BrdU for 18 h following fixation and staining.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169