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Brk antibody

Anti-Brk antibody used in Western Blot (WB). GTX33043
Anti-Brk antibody used in IHC (Paraffin sections) (IHC-P). GTX33043
Anti-Brk antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33043

Cat. No. GTX33043

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Brk products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-451 of human PTK6 (NP_005966.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 6 , BRK

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Brk antibody used in Western Blot (WB). GTX33043

GTX33043 WB Image

WB analysis of various sample lysates using GTX33043 Brk antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Brk antibody used in IHC (Paraffin sections) (IHC-P). GTX33043

GTX33043 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33043 Brk antibody.
Dilution : 1:100

Anti-Brk antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33043

GTX33043 ICC/IF Image

ICC/IF analysis of A549 cells using GTX33043 Brk antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399