GeneTex
United States (US)

Brk antibody

GTX33043_20180530_ICCIF_18121410_164.jpg
GTX33043_20180530_WB_18121410_882.jpg
GTX33043_20180530_IHC-P_18121410_449.jpg

Cat No. GTX33043

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-451 of human PTK6 (NP_005966.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Kinase 6,Brk,Ptk6

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX33043_20180530_ICCIF_18121410_164.jpg

GTX33043 ICC/IF Image

ICC/IF analysis of A549 cell using GTX33043 Brk antibody.
Blue : DAPI

GTX33043_20180530_WB_18121410_882.jpg

GTX33043 WB Image

WB analysis of various samples using GTX33043 Brk antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX33043_20180530_IHC-P_18121410_449.jpg

GTX33043 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33043 Brk antibody.
Dilution : 1:100

Package List Price ($)
$ 329