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Brn2 antibody

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31506
Anti-Brn2 antibody used in Western Blot (WB). GTX31506

Cat No. GTX31506

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , 3T3 (NIH) Cell Lysate(GTX27179)

Specificity/Sensitivity

At least two isoforms are known to exist. This antibody will recognize both isoforms. This antibody is predicted to not cross-react with other members of the POU domain class 3 family.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal Brn2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human Brn2.The immunogen is located within the last 50 amino acids of Brn2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 3 homeobox 2 , BRN2 , N-Oct3 , OCT7 , OTF-7 , OTF7 , POUF3 , brn-2 , oct-7

Cellular Localization

Nucleus

Background

This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-Brn2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31506

GTX31506 IHC-P Image

IHC-P analysis of human brain tissue using GTX31506 Brn2 antibody.
Working concentration : 20 μg/ml

Anti-Brn2 antibody used in Western Blot (WB). GTX31506

GTX31506 WB Image

WB analysis of 3T3 cell lysate using GTX31506 Brn2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339