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C/EBP alpha antibody [4E10]

Anti-C/EBP alpha antibody [4E10] used in ELISA (ELISA). GTX60578
Anti-C/EBP alpha antibody [4E10] used in Flow cytometry (FACS). GTX60578
Anti-C/EBP alpha antibody [4E10] used in Western Blot (WB). GTX60578
Anti-C/EBP alpha antibody [4E10] used in Western Blot (WB). GTX60578

Cat. No. GTX60578

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E10

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all C/EBP alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CEBPA expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP

Cellular Localization

Nucleus

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-C/EBP alpha antibody [4E10] used in ELISA (ELISA). GTX60578

GTX60578 ELISA Image

ELISA analysis of antigen using GTX60578 C/EBP alpha antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-C/EBP alpha antibody [4E10] used in Flow cytometry (FACS). GTX60578

GTX60578 FACS Image

FACS analysis of HeLa cells using GTX60578 C/EBP alpha antibody [4E10].
Green : C/EBP alpha
Red : negative control

Anti-C/EBP alpha antibody [4E10] used in Western Blot (WB). GTX60578

GTX60578 WB Image

WB analysis of THP-1 cell lysate using GTX60578 C/EBP alpha antibody [4E10].

Anti-C/EBP alpha antibody [4E10] used in Western Blot (WB). GTX60578

GTX60578 WB Image

WB analysis of human C/EBP alpha (AA: 139-204) recombinant protein using GTX60578 C/EBP alpha antibody [4E10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409