C/EBP alpha antibody [5B7]

Cat. No. GTX60584

Host	Mouse
Clonality	Monoclonal
Clone Name	5B7
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

See all C/EBP alpha products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide of human CEBPA (AA: C-RKSRDKAKRNVETKV).

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP

Cellular Localization

Nucleus

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Database

Gene ID: 1050 CEBPA
UniProt: P49715 CEBPA

Research Area

DATA IMAGES

GTX60584 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60584 C/EBP alpha antibody [5B7].
Green : C/EBP alpha
Red: Actin filaments

GTX60584 WB Image

WB analysis of Jurkat (1), k562 (2), and HepG2 (3) cell lysate using GTX60584 C/EBP alpha antibody [5B7].

GTX60584 ELISA Image

ELISA analysis of antigen using GTX60584 C/EBP alpha antibody [5B7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60584 IHC-P Image

IHC-P analysis of human rectum tissue using GTX60584 C/EBP alpha antibody [5B7].

GTX60584 FACS Image

FACS analysis of MCF-7 cells using GTX60584 C/EBP alpha antibody [5B7].
Green : C/EBP alpha
Red : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty