APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Synthesized peptide of human CEBPA (AA: C-RKSRDKAKRNVETKV).
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP
Cellular Localization
Nucleus
Background
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Database
Research Area
DATA IMAGES
|
GTX60584 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60584 C/EBP alpha antibody [5B7]. Green : C/EBP alpha Red: Actin filaments
|
|
GTX60584 WB Image
WB analysis of Jurkat (1), k562 (2), and HepG2 (3) cell lysate using GTX60584 C/EBP alpha antibody [5B7].
|
|
GTX60584 ELISA Image
ELISA analysis of antigen using GTX60584 C/EBP alpha antibody [5B7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
|
|
GTX60584 IHC-P Image
IHC-P analysis of human rectum tissue using GTX60584 C/EBP alpha antibody [5B7].
|
|
GTX60584 FACS Image
FACS analysis of MCF-7 cells using GTX60584 C/EBP alpha antibody [5B7]. Green : C/EBP alpha Red : negative control
|
REFERENCE
There are currently no references for C/EBP alpha antibody [5B7] (GTX60584). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for C/EBP alpha antibody [5B7] (GTX60584). Be the first to share your experience with this product.