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C/EBP alpha antibody [HL1747]

Recombinant antibody Orthogonal
Anti-C/EBP alpha antibody [HL1747] used in Western Blot (WB). GTX637397
Anti-C/EBP alpha antibody [HL1747] used in Western Blot (WB). GTX637397
Anti-C/EBP alpha antibody [HL1747] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637397

Cat. No. GTX637397

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1747

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all C/EBP alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Mouse, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP

Cellular Localization

Nucleus

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-C/EBP alpha antibody [HL1747] used in Western Blot (WB). GTX637397

GTX637397 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (GTX637397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-C/EBP alpha antibody [HL1747] used in Western Blot (WB). GTX637397

GTX637397 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (GTX637397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-C/EBP alpha antibody [HL1747] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637397

GTX637397 ICC/IF Image

C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (GTX637397) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations