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C/EBP alpha antibody [N1], N-term

Anti-C/EBP alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX100674
Anti-C/EBP alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX100674
Anti-C/EBP alpha antibody [N1], N-term used in Western Blot (WB). GTX100674
Anti-C/EBP alpha antibody [N1], N-term used in Western Blot (WB). GTX100674

Cat No. GTX100674

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

THP-1 , HL-60 , C2C12 , *HepG2 , *NB4 , *U937 , *THP-1 , *MV4-11

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCAAT enhancer binding protein alpha,C/EBP-alpha,CEBP

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-C/EBP alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX100674

GTX100674 IP Image

C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-C/EBP alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX100674

GTX100674 ChIP assay Image

Cross-linked ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
ChIP experiment and primer designs are based on FASEB J. 2005 Jul;19(9):1067-77.

Anti-C/EBP alpha antibody [N1], N-term used in Western Blot (WB). GTX100674

GTX100674 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C/EBP alpha antibody [N1], N-term used in Western Blot (WB). GTX100674

GTX100674 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE
REVIEW

C/EBP alpha antibody [N1], N-term Cat No. GTX100674

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 06-Dec-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : NB4 cells,U937 cells,THP-1 cells,MV4-11 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 28-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 100μg
Blocking : 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 24Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169