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C/EBP alpha (phospho Ser21) antibody

Anti-C/EBP alpha (phospho Ser21) antibody used in IHC (Paraffin sections) (IHC-P). GTX55304
Anti-C/EBP alpha (phospho Ser21) antibody used in Western Blot (WB). GTX55304

Cat. No. GTX55304

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from human C/EBP alpha.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP

Cellular Localization

Nucleus

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]

Database

Research Area

DATA IMAGES

Anti-C/EBP alpha (phospho Ser21) antibody used in IHC (Paraffin sections) (IHC-P). GTX55304

GTX55304 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55304 C/EBP alpha (phospho Ser21) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-C/EBP alpha (phospho Ser21) antibody used in Western Blot (WB). GTX55304

GTX55304 WB Image

WB analysis of extracts from HepG2 cells treated with EGF using GTX55304 C/EBP alpha (phospho Ser21) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for C/EBP alpha (phospho Ser21) antibody (GTX55304). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for C/EBP alpha (phospho Ser21) antibody (GTX55304). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399