Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/ml |
4 μg/ml |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects the LAP*, LAP and LIP isoforms of C/EBP beta.
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Recombinant mouse LIP.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_369010
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CCAAT/enhancer binding protein (C/EBP), beta , C/EBPbeta , CRP2 , IL-6DBP , LAP , LIP , NF-IL6 , NF-M , Nfil6
Cellular Localization
Nucleus,Cytoplasm
Background
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues.? These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.? One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells.? Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation.? From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
Database
Research Area