GeneTex
United States (US)

C/EBP beta antibody [1H7]

GTX15050_252_ICC-IF_19011519_553.jpg

Cat No. GTX15050

Host Mouse
Clonality Monoclonal
Clone Name 1H7
Isotype IgG
Application WB, ICC/IF, IP, ChIP assay, GSA
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF 4 μg/ml
IP Assay dependent
ChIP assay Assay dependent
GSA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

This antibody detects the LAP*, LAP and LIP isoforms of C/EBP beta.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse LIP.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ccaat/Enhancer Binding Protein (C/Ebp), Beta,C/Ebpbeta,Crp2,Il-6Dbp,Lap,Lip,Nf-Il6,Nf-M,Nfil6,Cebpb

Cellular Localization

Cytoplasm,Nucleus

Background

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues.? These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.? One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells.? Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation.? From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

Database

Research Area

DATA IMAGES
GTX15050_252_ICC-IF_19011519_553.jpg

GTX15050 ICC/IF Image

ICC/IF analysis of P388D1 cells using GTX15050 C/EBP beta antibody [1H7].

REFERENCE
Package List Price ($)
$ 289