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C/EBP beta antibody [47A1]

Anti-C/EBP beta antibody [47A1] used in Western Blot (WB). GTX50067

Cat. No. GTX50067

Host

Mouse

Clonality

Monoclonal

Clone Name

47A1

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human C/EBP-beta (1-271 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein beta , C/EBP-beta , IL6DBP , NF-IL6 , TCF5

Cellular Localization

Nucleus , Cytoplasm

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody [47A1] used in Western Blot (WB). GTX50067

GTX50067 WB Image

NIH 3T3 cell lysates(30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399