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C/EBP beta antibody [47A1]

Anti-C/EBP beta antibody [47A1] used in Western Blot (WB). GTX50067

Cat No. GTX50067

Host

Mouse

Clonality

Monoclonal

Clone Name

47A1

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human C/EBP-beta (1-271 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ccaat Enhancer Binding Protein Beta , C/Ebp-Beta , Il6Dbp , Nf-Il6 , Tcf5 , Cebpb

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPalpha, C/EBP?, C/EBP?, C/EBPepsilon, and C/EBPgamma . Among these proteins, C/EBPbeta is usually highly expressed in liver, intestine, lung and adipose. C/EBPbeta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody [47A1] used in Western Blot (WB). GTX50067

GTX50067 WB Image

NIH 3T3 cell lysates(30?g) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-? antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf