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C/EBP beta antibody

Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675
Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675
Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675
Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675
Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675

Cat No. GTX100675

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949960

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CCAAT enhancer binding protein beta , C/EBP-beta , IL6DBP , NF-IL6 , TCF5

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675

GTX100675 IP Image

Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 μg of C/EBP beta antibody (GTX100675).
Western blot analysis was performed using C/EBP beta antibody (GTX100675) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675

GTX100675 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP beta antibody (GTX100675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675

GTX100675 ICC/IF Image

C/EBP beta antibody detects C/EBP beta protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C/EBP beta stained by C/EBP beta antibody (GTX100675) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675

GTX100675 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP beta antibody. The precipitated DNA was detected by PCR with primer set targeting to CR2pD.
ChIP experiment and primer designs are based on Mol Immunol. 2009 Aug;46(13):2613-22.

Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675

GTX100675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta(GTX100675) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

C/EBP beta antibody Cat No. GTX100675

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26046540
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29875371
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169