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C/EBP beta antibody

Anti-C/EBP beta antibody used in Western Blot (WB). GTX64382
Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX64382
Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX64382

Cat. No. GTX64382

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all C/EBP beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:20 - 1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human CEBPB (NP_005185.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888596

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein beta , C/EBP-beta , IL6DBP , NF-IL6 , TCF5

Cellular Localization

Nucleus,Cytoplasm

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody used in Western Blot (WB). GTX64382

GTX64382 WB Image

WB analysis of various sample lysates using GTX64382 C/EBP beta antibody.
Dilution : 1:700
Loading : 25μg per lane

Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX64382

GTX64382 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64382 C/EBP beta antibody.
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:100

Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX64382

GTX64382 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64382 C/EBP beta antibody.
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.0
Dilution : 1:100

REFERENCE

Application Reference

Chen CC et al. Transl Res 2019; (Epub) Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399