C/EBP beta (phospho Thr235) antibody

Cat. No. GTX55008

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX55008 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
ICC/IF	1:100 - 1:500
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of C/EBP beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein beta , C/EBP-beta , IL6DBP , NF-IL6 , TCF5

Cellular Localization

Nucleus,Cytoplasm

Background

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

Database

Gene ID: 1051 CEBPB
UniProt: P17676 CEBPB

Research Area

DATA IMAGES

GTX55008 WB Image

WB analysis of EGF-treated HEK293T (A), EGF-treated COLO205 (B) whole cell lysates using GTX55008 C/EBP beta (phospho Thr235) antibody.

GTX55008 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX55008 C/EBP beta (phospho Thr235) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX55008 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55008 C/EBP beta (phospho Thr235) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty