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C/EBP delta antibody

KO/KD Orthogonal Overexpression
Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713
Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713
Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713

Cat. No. GTX48713

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA, IHC (Free Floating)

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all C/EBP delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:15000
IP 0.8-1.5 μg/mL
ELISA 1:4000-1:9000
IHC (Free Floating) 1:2000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein corresponding to the amino terminus of mouse C/EBP.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT/enhancer binding protein (C/EBP), delta , c/EBPdelta

Cellular Localization

Nucleus

Background

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Database

Research Area

DATA IMAGES

Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713

GTX48713 WB Image

Overexpression

WB analysis of transfected and non-transfected whole cell lysate using GTX48713 C/EBP delta antibody.
Lane 1 : Human C/EBP beta
Lane 2 : Mouse C/EBP delta
Lane 3 : Mock
Lane 4 : Human C/EBP delta
Loading : 35 μg
Dilution : 1:1500

Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713

GTX48713 WB Image

KO/KD Orthogonal

WB analysis of LPS-treated and untreated mouse primary macrophage lysate using GTX48713 C/EBP delta antibody.
Lane 1 : Wild-type
Lane 2 : Wild-type cells treated with LPS
Lane 3 : C/EBP delta knockout cells treated with LPS
Loading : 35 μg
Dilution : 1:1500

Anti-C/EBP delta antibody used in Western Blot (WB). GTX48713

GTX48713 WB Image

KO/KD Orthogonal

Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 μg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4ºC Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399