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C-Peptide antibody [HL1158]

Recombinant antibody Orthogonal
Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462
Anti-C-Peptide antibody [HL1158] used in ELISA (ELISA). GTX636462
Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462
Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462

Cat. No. GTX636462

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1158

Isotype

 IgG

Application

 IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C-Peptide products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:10000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to human c-peptide.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909984

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462

GTX636462 IHC-P Image

C-Peptide antibody [HL1158] detects secreted C-Peptide protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C-Peptide antibody [HL1158] used in ELISA (ELISA). GTX636462

GTX636462 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (19.05-0.3 nM). Coated protein was probed with C-Peptide antibody [HL1158] (GTX636462) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462

GTX636462 IHC-P Image

C-Peptide antibody [HL1158] detects secreted C-Peptide protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C-Peptide antibody [HL1158] used in IHC (Paraffin sections) (IHC-P). GTX636462

GTX636462 IHC-P Image

C-Peptide antibody [HL1158] detects secreted C-Peptide protein by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas.
C-Peptide stained by C-Peptide antibody [HL1158] (GTX636462) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations