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C-Peptide antibody

Comparison
Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708
Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708
Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708
Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708

Cat. No. GTX129708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C-Peptide products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human C-Peptide. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886073

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IRDN Antibody , MODY10 Antibody , IDDM2 Antibody , INS Antibody , ILPR Antibody , insulin Antibody

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708

GTX129708 IHC-P Image

C-Peptide antibody detects C-Peptide protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
C-Peptide stained by C-Peptide antibody (GTX129708) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708

GTX129708 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (200-3.125 ng/mL). Coated protein was probed with C-Peptide antibody (GTX129708) (0.5 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708

GTX129708 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating plate with 50 μL of mature C-Peptide protein at concentrations ranging from 3.125 ng/mL to 200 ng/mL. The coated protein is detected with C-Peptide antibody (GTX129708) or competitor's antibody (MAB14171) at 0.5 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708

GTX129708 IHC-P Image

C-Peptide antibody detects C-Peptide protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
C-Peptide stained by C-Peptide antibody (GTX129708) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169