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C-Peptide antibody

Comparison
Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708
Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708
Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708
Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708

Cat. No. GTX129708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C-Peptide products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human C-Peptide. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886073

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IRDN Antibody , MODY10 Antibody , IDDM2 Antibody , INS Antibody , ILPR Antibody , insulin Antibody

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708

GTX129708 IHC-P Image

C-Peptide antibody detects C-Peptide protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
C-Peptide stained by C-Peptide antibody (GTX129708) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C-Peptide antibody used in IHC (Paraffin sections) (IHC-P). GTX129708

GTX129708 IHC-P Image

C-Peptide antibody detects C-Peptide protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
C-Peptide stained by C-Peptide antibody (GTX129708) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708

GTX129708 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant mature C-Peptide protein (200-3.125 ng/mL). Coated protein was probed with C-Peptide antibody (GTX129708) (0.5 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-C-Peptide antibody used in ELISA (ELISA). GTX129708

GTX129708 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating plate with 50 μL of mature C-Peptide protein at concentrations ranging from 3.125 ng/mL to 200 ng/mL. The coated protein is detected with C-Peptide antibody (GTX129708) or competitor's antibody (MAB14171) at 0.5 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169