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C Reactive Protein antibody [N1C3]

Anti-C Reactive Protein antibody [N1C3] used in ELISA (ELISA). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

Cat. No. GTX101262

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all C Reactive Protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

H1299 , Molt-4 , rat plasma

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C Reactive Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950040

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-reactive protein , PTX1

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C Reactive Protein antibody [N1C3] used in ELISA (ELISA). GTX101262

GTX101262 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human C Reactive Protein, His tag (GTX138453-pro) (72.07-2.25 nM). Coated protein was probed with C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262

GTX101262 IHC-P Image

C Reactive Protein antibody [N1C3] detects C Reactive Protein protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000.

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Molt-4 (GTX27912)
12% SDS PAGE
GTX101262 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169