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C Reactive Protein antibody [N1C3]

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in ELISA (ELISA). GTX101262
Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262

Cat. No. GTX101262

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Human kidney , rat plasma

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C Reactive Protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-reactive protein , PTX1

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Molt-4 (GTX27912)
12% SDS PAGE
GTX101262 diluted at 1:1000

Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262

GTX101262 IHC-P Image

C Reactive Protein antibody [N1C3] detects secreted C Reactive Protein protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
C Reactive Protein stained by C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-C Reactive Protein antibody [N1C3] used in Western Blot (WB). GTX101262

GTX101262 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:250000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C Reactive Protein antibody [N1C3] used in ELISA (ELISA). GTX101262

GTX101262 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human C Reactive Protein, His tag (GTX138453-pro) (144.14-2.25 nM). Coated protein was probed with C Reactive Protein antibody [N1C3] (GTX101262) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-C Reactive Protein antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101262

GTX101262 IHC-P Image

C Reactive Protein antibody [N1C3] detects C Reactive Protein protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
C Reactive Protein antibody [N1C3] (GTX101262) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169