GeneTex
United States (US)

C1 inhibitor antibody [N1C3]

GTX105316_39974_20160512_WB_H_plasma_19011506_544.jpg
GTX105316_39974_20160519_WB_19011506_714.jpg
GTX105316_39974_IHC_19011506_478.jpg

Cat No. GTX105316

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , human plasma
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C1 inhibitor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1IN antibody, C1INH antibody, C1NH antibody, HAE1 antibody, HAE2 antibody, SERPING1 antibody, serpin G1 antibody, serine/cysteine proteinase inhibitor clade G member 1 antibody, C1 esterase inhibitor antibody, complement component 1 inhibitor antibody, plasma protease C1 inhibitor antibody, C1-inhibiting factor antibody, "serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 antibody"

Cellular Localization

Secreted

Background

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105316_39974_20160512_WB_H_plasma_19011506_544.jpg

GTX105316 WB Image

Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C1 inhibitor antibody [N1C3] (GTX105316) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105316_39974_20160519_WB_19011506_714.jpg

GTX105316 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C1 inhibitor antibody [N1C3] (GTX105316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105316_39974_IHC_19011506_478.jpg

GTX105316 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using C1 inhibitor(GTX105316) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf