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C1 inhibitor antibody [N1C3]

Anti-C1 inhibitor antibody [N1C3] used in Western Blot (WB). GTX105316
Anti-C1 inhibitor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105316

Cat No. GTX105316

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Bovine
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , human plasma
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C1 inhibitor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

serpin family G member 1 , C1IN , C1INH , C1NH , HAE1 , HAE2

Cellular Localization

Secreted

Background

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]

Database

  • Gene ID: 710 SERPING1
  • UniProt: P05155 SERPING1

Research Area

DATA IMAGES
Anti-C1 inhibitor antibody [N1C3] used in Western Blot (WB). GTX105316

GTX105316 WB Image

Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C1 inhibitor antibody [N1C3] (GTX105316) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C1 inhibitor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105316

GTX105316 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using C1 inhibitor(GTX105316) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf