GeneTex
United States (US)

C11orf54 antibody

GTX107093_41003_IFA_19011506_734.jpg
GTX107093_41003_WB_19011506_759.jpg

Cat No. GTX107093

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C11orf54. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Chromosome 11 Open Reading Frame 54,Ptd012,Ptod012,C11Orf54

Cellular Localization

Nucleus

Background

Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.

Database

DATA IMAGES
GTX107093_41003_IFA_19011506_734.jpg

GTX107093 ICC/IF Image

C11orf54 antibody detects C11orf54 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: C11orf54 protein stained by C11orf54 antibody (GTX107093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX107093_41003_WB_19011506_759.jpg

GTX107093 WB Image

C11orf54 antibody detects C11orf54 protein by Western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
C. 30 µg HCT116 whole cell lysate/extract
12 % SDS-PAGE
C11orf54 antibody (GTX107093) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169