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C1D antibody

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439
Anti-C1D antibody used in Western Blot (WB). GTX129439
Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439
Anti-C1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129439

Cat. No. GTX129439

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all C1D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human C1D Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885996

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C1D nuclear receptor corepressor , LRP1 , Rrp47 , SUN-CoR , SUNCOR , hC1D

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

The protein encoded by this gene is a DNA binding and apoptosis-inducing protein and is localized in the nucleus. It is also a Rac3-interacting protein which acts as a corepressor for the thyroid hormone receptor. This protein is thought to regulate TRAX/Translin complex formation. Several alternatively spliced transcript variants of this gene have been described, but the full length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439

GTX129439 IHC-P Image

C1D antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
C1D antibody (GTX129439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C1D antibody used in Western Blot (WB). GTX129439

GTX129439 WB Image

C1D antibody detects C1D protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
C1D antibody (GTX129439) dilution: 1:1000

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439

GTX129439 IHC-P Image

C1D antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
C1D antibody (GTX129439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129439

GTX129439 ICC/IF Image

C1D antibody detects C1D protein at nucleus, nucleolus and cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C1D protein stained by C1D antibody (GTX129439) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Tomita T et al. J Biochem 2018; (Epub) C1D is not directly involved in the repair of UV-damaged DNA but protects cells from oxidative stress by regulating gene expressions in human cell lines.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169