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C1D antibody

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439
Anti-C1D antibody used in Western Blot (WB). GTX129439
Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439
Anti-C1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129439

Cat No. GTX129439

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human C1D Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C1D nuclear receptor corepressor , LRP1 , Rrp47 , SUN-CoR , SUNCOR , hC1D

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

The protein encoded by this gene is a DNA binding and apoptosis-inducing protein and is localized in the nucleus. It is also a Rac3-interacting protein which acts as a corepressor for the thyroid hormone receptor. This protein is thought to regulate TRAX/Translin complex formation. Several alternatively spliced transcript variants of this gene have been described, but the full length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439

GTX129439 IHC-P Image

C1D antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
C1D antibody (GTX129439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C1D antibody used in Western Blot (WB). GTX129439

GTX129439 WB Image

C1D antibody detects C1D protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
C1D antibody (GTX129439) dilution: 1:1000

Anti-C1D antibody used in IHC (Paraffin sections) (IHC-P). GTX129439

GTX129439 IHC-P Image

C1D antibody detects C1D protein at nucleolus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
C1D antibody (GTX129439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C1D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129439

GTX129439 ICC/IF Image

C1D antibody detects C1D protein at nucleus, nucleolus and cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C1D protein stained by C1D antibody (GTX129439) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169