GeneTex
United States (US)

C1q antibody

Cat No. GTX28779

Host Sheep
Clonality Polyclonal
Isotype IgG
Application DID, RID
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
DID Assay dependent
RID Neat-1:2

Note :

DID
10 μl antiserum vs 20 μl fresh plasma

RID
0.1 μl antiserum / cm2 gel vs. 20 μl fresh plasma Neat-1:2

Not tested in other applications.

Specificity/Sensitivity

Gives a single arc when tested by IEP against fresh human plasma. Identity has been confirmed bydouble diffusion (Ouchterlony) against fresh human plasma and a known anti-human C1q. C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with thetetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and iscomposed of three basic subunits termed A, B and C (27,550, 25,200, 23,800 Da-respectively) whichassociate in the proportion 6 A-B subunits (52,750 Da) and 3 C-C subunits (47,600 Da), to form wholeC1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, whichare also bound to C1q and so initiates the classical pathway of complement activation. The normalplasma concentration of C1q is 100-180 mg/L.
PROPERTIES

Form

Liquid

Buffer

Glycine Buffered Saline pH 7.4, 0.099% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1 mM EDTA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

5.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human C1q, > 95% pure (SDS-PAGE). Prepared from human plasma.

Purification

IgG fraction
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Package List Price ($)
$ 339