Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Neat-1:2 |
Note :
RID
0.1 μl antiserum / cm² gel vs. 20 μl fresh plasma Neat-1:2
|
Not tested in other applications.
Product Note
Gives a single arc when tested by IEP against fresh human plasma. Identity has been confirmed bydouble diffusion (Ouchterlony) against fresh human plasma and a known anti-human C1q. C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with thetetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and iscomposed of three basic subunits termed A, B and C (27,550, 25,200, 23,800 Da-respectively) whichassociate in the proportion 6 A-B subunits (52,750 Da) and 3 C-C subunits (47,600 Da), to form wholeC1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, whichare also bound to C1q and so initiates the classical pathway of complement activation. The normalplasma concentration of C1q is 100-180 mg/L.
Form
Liquid
Buffer
Glycine buffered Saline pH7.4, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA
Preservative
0.099% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
5.07 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human C1q, > 95% pure (SDS-PAGE). Prepared from human plasma.
Purification
IgG fraction
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.