*Optimal dilutions/concentrations should be determined by the researcher.
10 μl antiserum vs 20 μl fresh plasma
0.1 μl antiserum / cm2 gel vs. 20 μl fresh plasma Neat-1:2
Not tested in other applications.
Gives a single arc when tested by IEP against fresh human plasma. Identity has been confirmed bydouble diffusion (Ouchterlony) against fresh human plasma and a known anti-human C1q. C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with thetetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and iscomposed of three basic subunits termed A, B and C (27,550, 25,200, 23,800 Da-respectively) whichassociate in the proportion 6 A-B subunits (52,750 Da) and 3 C-C subunits (47,600 Da), to form wholeC1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, whichare also bound to C1q and so initiates the classical pathway of complement activation. The normalplasma concentration of C1q is 100-180 mg/L.
Glycine Buffered Saline pH 7.4, 0.099% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1 mM EDTA
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
5.07 mg/ml (Please refer to the vial label for the specific concentration.)
Human C1q, > 95% pure (SDS-PAGE). Prepared from human plasma.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.