GeneTex
United States (US)

C1qA antibody

GTX112883_40114_20160324_WB_H_plasma_18121410_545.jpg
GTX112883_40114_20160602_IFA_18121410_994.jpg
GTX112883_40114_WB_M_spleen_18121410_712.jpg

Cat No. GTX112883

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

human plasma , mouse spleen
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C1qA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Complement C1Q A Chain,C1Qa

Cellular Localization

Secreted

Background

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112883_40114_20160324_WB_H_plasma_18121410_545.jpg

GTX112883 WB Image

Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1qA antibody (GTX112883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112883_40114_20160602_IFA_18121410_994.jpg

GTX112883 ICC/IF Image

C1qA antibody detects C1qA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: C1qA protein stained by C1qA antibody (GTX112883) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX112883_40114_WB_M_spleen_18121410_712.jpg

GTX112883 WB Image

C1qA antibody detects C1QA protein by western blot analysis.
A. 50 µg mouse spleen lysate/extract
12% SDS-PAGE
C1qA antibody (GTX112883) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169