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C1r antibody

Anti-C1r antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104350
Anti-C1r antibody used in Western Blot (WB). GTX104350
Anti-C1r antibody used in Western Blot (WB). GTX104350
Anti-C1r antibody used in Western Blot (WB). GTX104350
Anti-C1r antibody used in Western Blot (WB). GTX104350

Cat No. GTX104350

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C1r. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

complement C1r , EDSPD1

Background

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Database

Research Area

DATA IMAGES
Anti-C1r antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104350

GTX104350 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HCT116, using C1r(GTX104350) antibody at 1:500 dilution.

Anti-C1r antibody used in Western Blot (WB). GTX104350

GTX104350 WB Image

Human plasma (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C1r antibody used in Western Blot (WB). GTX104350

GTX104350 WB Image

Mouse plasma (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C1r antibody used in Western Blot (WB). GTX104350

GTX104350 WB Image

Rat plasma (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C1r antibody used in Western Blot (WB). GTX104350

GTX104350 WB Image

Human plasma (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 95 kDa, 60 kDa, and 34 kDa. It is possibly due to alternative splicing.
Reference:PMID: 26808924

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf