Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HCT116, using C1r(GTX104350) antibody at 1:500 dilution.

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1r antibody (GTX104350) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.