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C1s antibody

Anti-C1s antibody used in IHC (Paraffin sections) (IHC-P). GTX105464
Anti-C1s antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105464
Anti-C1s antibody used in Western Blot (WB). GTX105464
Anti-C1s antibody used in Western Blot (WB). GTX105464

Cat No. GTX105464

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

293T , HepG2 culture medium pellet , HepG2 culture medium supernatant

Predict Reactivity

Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C1s. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949782

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

complement C1s , EDSPD2

Background

This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C1s antibody used in IHC (Paraffin sections) (IHC-P). GTX105464

GTX105464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C1s(GTX105464) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C1s antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105464

GTX105464 ICC/IF Image

C1s antibody detects C1S protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: C1S protein stained by C1s antibody (GTX105464) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-C1s antibody used in Western Blot (WB). GTX105464

GTX105464 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C1s antibody (GTX105464) diluted at 1:1000.

Anti-C1s antibody used in Western Blot (WB). GTX105464

GTX105464 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C1s antibody (GTX105464) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169