C20orf7 antibody [N3C3]

Cat. No. GTX119214

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all C20orf7 products

Datasheet
Document

View product citations for antibody GTX119214 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C20orf7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724480

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase complex assembly factor 5 , C20orf7 , MC1DN16 , bA526K24.2 , dJ842G6.1

Cellular Localization

Mitochondrion inner membrane

Background

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 79133 NDUFAF5
UniProt: Q5TEU4 NDUFAF5

Research Area

DATA IMAGES

GTX119214 ICC/IF Image

C20orf7 antibody [N3C3] detects C20orf7 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: C20orf7 protein stained by C20orf7 antibody [N3C3] (GTX119214) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX119214 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX119214 diluted at 1:2000

GTX119214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using C20orf7(GTX119214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty