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C21orf2 antibody [N1C2]

Anti-C21orf2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119046
Anti-C21orf2 antibody [N1C2] used in Western Blot (WB). GTX119046
Anti-C21orf2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119046

Cat No. GTX119046

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C21orf2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cilia And Flagella Associated Protein 410,C21Orf2,Lrrc76,Rdms,Smdax,Yf5/A2,Cfap410

Database

DATA IMAGES
Anti-C21orf2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119046

GTX119046 ICC/IF Image

C21orf2 antibody [N1C2] detects C21orf2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C21orf2 stained by C21orf2 antibody [N1C2] (GTX119046) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-C21orf2 antibody [N1C2] used in Western Blot (WB). GTX119046

GTX119046 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX119046 diluted at 1:500

Anti-C21orf2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119046

GTX119046 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using C21orf2(GTX119046) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169