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C21orf33 antibody

Anti-C21orf33 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101483
Anti-C21orf33 antibody used in Western Blot (WB). GTX101483
Anti-C21orf33 antibody used in IHC (Paraffin sections) (IHC-P). GTX101483

Cat. No. GTX101483

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all C21orf33 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

A549 , HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C21orf33. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167415

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamine amidotransferase like class 1 domain containing 3A , C21orf33 , ES1 , GATD3 , GT335 , HES1 , KNPH , KNPI

Cellular Localization

Mitochondrion

Background

This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C21orf33 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101483

GTX101483 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using KNP-I(GTX101483) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-C21orf33 antibody used in Western Blot (WB). GTX101483

GTX101483 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HepG2
12% SDS PAGE
GTX101483 diluted at 1:1000

Anti-C21orf33 antibody used in IHC (Paraffin sections) (IHC-P). GTX101483

GTX101483 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KNP-I(GTX101483) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169