GeneTex
  • Country / Location Selection

United States (US)

C3 antibody [C3], C-term

Anti-C3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101316
Anti-C3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101316
Anti-C3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX101316
Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316
Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316
Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316

Cat No. GTX101316

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, Immunosubtraction

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Immunosubtraction Assay dependent
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

Positive Control

Huh7 , HepG2

Specificity/Sensitivity

The mouse reactivity of this antibody is based on mouse IHC-P testing and may be not suitable for mouse WB assay.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

complement C3 , AHUS5 , ARMD9 , ASP , C3a , C3b , CPAMD1 , HEL-S-62p

Cellular Localization

Secreted

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101316

GTX101316 IHC-P Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C3 antibody [C3], C-term (GTX101316) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101316

GTX101316 ICC/IF Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-C3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX101316

GTX101316 IP Image

Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316).
Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316

GTX101316 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at 1:20000.

Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316

GTX101316 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C3 antibody [C3], C-term used in Western Blot (WB). GTX101316

GTX101316 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

There are currently no reviews for C3 antibody [C3], C-term (GTX101316). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf