GeneTex
United States (US)

C3 antibody [C3], C-term

GTX101316_42116_20151005_IHC-P_M_19011506_457.jpg
GTX101316_42116_20150924_IFA_19011506_800.jpg
GTX101316_42081_20150521_WB_19011506_675.jpg
GTX101316_41710_20150209_IP_19011506_118.jpg

Cat No. GTX101316

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, Immunosubtraction
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Immunosubtraction Assay dependent
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

Positive Control

Huh7 , HepG2

Specificity/Sensitivity

The mouse reactivity of this antibody is based on mouse IHC-P testing and may be not suitable for mouse WB assay.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Complement C3,Ahus5,Armd9,Asp,C3A,C3B,Cpamd1,Hel-S-62P,C3

Cellular Localization

Secreted

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101316_42116_20151005_IHC-P_M_19011506_457.jpg

GTX101316 IHC-P Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C3 antibody [C3], C-term (GTX101316) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101316_42116_20150924_IFA_19011506_800.jpg

GTX101316 ICC/IF Image

C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101316_42081_20150521_WB_19011506_675.jpg

GTX101316 WB Image

C3 antibody detects C3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody (GTX101316) diluted at a dilution of 1:1000.

GTX101316_41710_20150209_IP_19011506_118.jpg

GTX101316 IP Image

Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316).
Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169