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C3 antibody [HL1643]

Recombinant antibody
Anti-C3 antibody [HL1643] used in Western Blot (WB). GTX637224
Anti-C3 antibody [HL1643] used in Western Blot (WB). GTX637224
Anti-C3 antibody [HL1643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637224

Cat. No. GTX637224

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1643

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all C3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human C3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

complement C3 , AHUS5 , ARMD9 , ASP , C3a , C3b , CPAMD1 , HEL-S-62p

Cellular Localization

Secreted

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-C3 antibody [HL1643] used in Western Blot (WB). GTX637224

GTX637224 WB Image

Rat plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (GTX637224) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C3 antibody [HL1643] used in Western Blot (WB). GTX637224

GTX637224 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 antibody [HL1643] (GTX637224) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C3 antibody [HL1643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637224

GTX637224 ICC/IF Image

C3 antibody [HL1643] detects C3 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C3 stained by C3 antibody [HL1643] (GTX637224) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations