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C3 antibody

Anti-C3 antibody used in Western Blot (WB). GTX33050
Anti-C3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33050
Anti-C3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33050

Cat No. GTX33050

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

187 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3 (NP_000055.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

complement C3 , AHUS5 , ARMD9 , ASP , C3a , C3b , CPAMD1 , HEL-S-62p

Cellular Localization

Secreted

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-C3 antibody used in Western Blot (WB). GTX33050

GTX33050 WB Image

WB analysis of various samples using GTX33050 C3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-C3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33050

GTX33050 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33050 C3 antibody.
Dilution : 1:200

Anti-C3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33050

GTX33050 IHC-P Image

IHC-P analysis of rat liver tissue using GTX33050 C3 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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