C3a Receptor antibody [HL2831]

C3a Receptor antibody [HL2831] (GTX640102) detects C3a Receptor protein by flow cytometry analysis.
Sample: non-fixed and non-permeabilized HL-60 or HeLa cells were stained with 5μg/ml GTX640102 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4ºC for 1 hour.

Untreated (–) and treated (+) unboiled HL-60 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

C3a Receptor antibody [HL2831] detects C3a Receptor protein by immunohistochemical analysis.
Sample: Paraffin-embedded human cerebellum.
C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

C3a Receptor antibody [HL2831] detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded HL-60 xenograft.
C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

C3a Receptor antibody [HL2831] detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

C3a Receptor antibody [HL2831] detects C3a Receptor protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).